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Image Search Results
Journal: Molecules
Article Title: Effect of Immunomodulating Extract and Some Isolates from Etlingera rubroloba A.D. Poulsen Fruits on Diabetic Patients with Tuberculosis
doi: 10.3390/molecules28052401
Figure Lengend Snippet: Screening results of macrophage cell phagocytosis activity and IL-12 levels in each group.
Article Snippet:
Techniques: Activity Assay, Positive Control, Control
Journal: Molecules
Article Title: Effect of Immunomodulating Extract and Some Isolates from Etlingera rubroloba A.D. Poulsen Fruits on Diabetic Patients with Tuberculosis
doi: 10.3390/molecules28052401
Figure Lengend Snippet: Mean levels of IL-12 ± SEM of extracts and isolates from E.rubroloba fruit in each treatment group.
Article Snippet:
Techniques: Control
Journal: The American Journal of Gastroenterology
Article Title: Metagenomics Reveals Dysbiosis and a Potentially Pathogenic N. flavescens Strain in Duodenum of Adult Celiac Patients
doi: 10.1038/ajg.2016.95
Figure Lengend Snippet: N. flavescens ( Nf ) isolated from celiac disease (CD) patients induce a pro-inflammatory phenotype on dendritic cells. Filtered bacterial lysates from Neisseria flavescens ( Nf ), isolated from 5 active celiac patients (CD- Nf 1, 2, 3, 7, and 8), promoted the production of pro-inflammatory cytokines by dendritic cells (DCs). CD11c + DCs isolated from the spleens of C57B6 mice were cultured for 48 h in the presence of retinoic acid (RA) and transforming growth factor-β (TGF-β) with filtered Nf lysates from three active CD patients ( a ). All three tested bacterial lysates promoted the production of interleukin (IL)-12p40 by murine DCs, whereas untreated (stars upon horizontal bars), lipopolysaccharide (LPS)-treated (stars), and L GG-treated DCs did not ( b ). Human monocyte-derived DCs were cultured for 48 h in the presence of RA and TGF-β with filtered Nf lysates isolated from active CD patients ( c ). Tumor necrosis factor-α (TNF-α) levels were higher in supernatants of human DCs treated with Nf 1, Nf 7, and Nf 8 bacterial strains isolated from small intestinal biopsies of CD patients, and in those challenged with Nf 10 isolated from a pharyngeal swab of a control subject ( d ), than in those treated with bacterial LPS, used as a pro-inflammatory positive control (stars upon horizontal bars) and in untreated (UN) cells ( P <0.0001 for each of the treatments). IL-12p40 levels were higher in supernatants of human DCs challenged with Nf 1,2,3 filtered lysates than in those treated with bacterial LPS (stars) used as a pro-inflammatory positive control and in untreated (UN) cells (stars upon horizontal bars) ( e ). Dot plots represent values from three independent experiments. Each experiment was performed in duplicate. A two-way analysis of variance was performed to check for inter-group differences. * P <0.05, ** P <0.01, *** P <0.001, and **** P <0.0001.
Article Snippet: Forty-eight hours later, supernatants were harvested and tested by ELISA to check the levels of
Techniques: Isolation, Cell Culture, Derivative Assay, Control, Positive Control